Journal: Biomedicines

Article Title: Therapeutic Effects of Omentin-1 on Pulmonary Fibrosis by Attenuating Fibroblast Activation via AMP-Activated Protein Kinase Pathway

doi: 10.3390/biomedicines10112715

Figure Lengend Snippet: The effect of omentin-1 deficiency on BLM-induced lung fibrosis at 21 d. ( A ) Experimental design: BLM (1.0 mg/kg) was injected to induce fibrosis (21 days) in wild-type and Omentin-1 −/− mice. ( B ) Weight change of mice 21 days after BLM challenge ( n = 10). ( C ) Representative micrographs of H&E staining of lung tissue (Bar = 50 μm, n = 5). ( D ) Representative micrographs of Masson trichrome staining, immunohistochemical staining of lung sections for Collagen III (Col3) and α-SMA (Bar = 50 μm, n = 5). (E) Ashcroft score to grade fibrosis scale ( n = 5). ( F ) Collagen content was measured by hydroxyproline (HYP) assay ( n = 5). ( G ) The content of collagen III (Col3) and α-SMA in lung tissue detected by Western blotting with cropping gel at the relative molecular size. ( n = 3). ( H ) Semi-quantitative analysis of Col3 level evaluated by Western blotting. ( I ) Semi-quantitative analysis of α-SMA level evaluated by Western blotting. The data are presented as the mean ± SD. * p < 0.05, ** p < 0.01 vs. control group. # p < 0.05, ## p < 0.01 vs. BLM group.

Article Snippet: The cells were blocked with 5% BSA for 30 min, and stained overnight at 4 °C with the primary antibodies, i.e., mouse anti-α-SMA (1:100; Servicebio, Wuhan, China) and mouse anti- collagen III (1:100; Servicebio, Wuhan, China), followed by staining with Alexa Fluor 594-goat anti-mouse secondary antibodies (1:100, Beyotime, Shanghai, China) at room temperature for 30 min.

Techniques: Injection, Staining, Immunohistochemical staining, Western Blot, Control